《植物生理学报》 2010, 46(7): 701-706

不同理化因子对雨生红球藻CG-11 碳酸酐酶活性的影响

王铭, 桑敏, 李爱芬*, 张成武, 杨扬

暨南大学水生生物研究中心, 广州510632

通信作者：李爱芬；E-mail: tiger@jnu.edu.cn；Tel: 020-85224366

摘　要：

以雨生红球藻CG-11 为实验藻株, 探讨在不同CO2、HCO3-、Zn2+ 浓度以及pH 和氮磷比例条件下, 藻细胞的碳酸酐酶活性对这些理化因子的响应。结果表明, 通入空气实验组的碳酸酐酶活性最高, 为(75.20±1.53) U·mg-1 (Chl a), 通入5%CO2条件下的碳酸酐酶活性为(9.96±1.43) U·mg-1 (Chl a); 高浓度HCO3-对碳酸酐酶活性亦具有明显抑制作用, 培养液中可溶性无机碳的浓度与碳酸酐酶活性呈负相关; 在实验设置的pH 范围内, pH 9.0 时碳酸酐酶活性最高, 为(62.32±3.25) U·mg-1(Chl a); 适当的氮磷比与Zn2+浓度显著提高了雨生红球藻CG-11的生长速率, 碳酸酐酶的活性亦有明显提高。

关键词：雨生红球藻; 碳酸酐酶活性; 理化因子

收稿：2010-04-23   修定：2010-05-14

资助：珠海市科技重大项目(PB20041018)。

Effects of Physical and Chemical Factors on Carbonic Anhydrase Activity of Haematococcus pluvialis CG-11

WANG Ming, SANG Min, LI Ai-Fen*, ZHANG Cheng-Wu, YANG Yang

Institute of Hydrobiology, Jinan University, Guangzhou 510632, China

Corresponding author: LI Ai-Fen; E-mail: tiger@jnu.edu.cn; Tel: 020-85224366

Abstract:

The strain Haematococcus pluvialis CG-11 was used to explore the effects of different concentrations of CO₂, HCO₃^-, Zn²⁺, pH and the ratio of nitrogen and phosphorus on the carbonic anhydrase activity. The results showed that only supplying compressed air for mixing, the maximum carbonic anhydrase activity can reach (75.20±1.53) U·mg^-1 (Chl a), but providing compressed air enriched with 5% CO₂ for mixing, carbonic anhydrase activity was only (9.96±1.43) U·mg^-1 (Chl a). High concentration of HCO₃^- also inhibited the carbonic anhydrase activity, and the concentration of total inorganic carbon in culture medium and carbon anhydrase activity was negatively correlated. The maximum carbonic anhydrase activity was (62.32±3.25) U·mg^-1 (Chl a), that was obtained at pH 9.0 in the range of experiments. The appropriate ratio of nitrogen and phosphorus and the concentration of Zn²⁺ could significantly increase the growth rate of H. pluvialis CG-11, and carbonic anhydrase activity was also improved significantly.

Key words: Haematococcus pluvialis, carbonic anhydrase activity, physical and chemical factors